This museum is housed in the Kasbah (citadel built in the IXth century) where it occupies part of the premises. Like all regional museums, it contains only objects found in the city of Sousse and its vicinity, in particular funerary furnishing from to Punic tombs excavated in the very grounds of the Kasbah and that are displayed in the room known as that of the Tophet. The room also contains votive stelae that accompanied the sacrifices made to the Carthaginian divinities Baal and Tanit.

But because of the layout, the museum cannot be visited in chronological order. The visit begins with funerary plaques dating to the Christian period, sealed on the wall of a covered gallery that leads into a room containing two superb mosaics: one of horses in a stud farm and one of god Oceanus, who has remained in the collective memory of the inhabitants of Sousse under the name of Aoussou, a feast celebrated each year with great festivity.

Mosaics constitute the most important part of the museum containing a great number of pavements ranking amongst the most beautiful in Tunisia : Satyr and Bacchantes, the Triumph of Bacchus, the Abduction of Ganymede, the Triumph of Neptune, Venus adorning herself etc. Statuary, architectural decoration and everyday objects are also well represented.

The Khalf tower, overlooking the citadel provides a splendid view over the city.